Phagocytosis Assay Kit (Green Zymosan) (BN00651)

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BN00651
  • Phagocytosis Assay Kit (Green Zymosan) (BN00651)
  • Phagocytosis Assay Kit (Green Zymosan) (BN00651)
  • Phagocytosis Assay Kit (Green Zymosan) (BN00651)
€499

Description

ELISA Kit Technical ManualMSDS

Phagocytosis Assay Kit (Green Zymosan)

Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. Zymosan prepared from yeast cell wall (Saccharomyces cerevisiae), and consisting of protein-carbohydrate complexes is frequently used as a pathogen in phagocytosis assays. Assay Genie's Phagocytosis Assay Kit (Green Zymosan) utilizes pre-labeled Zymosan particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. Our kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptors ligands (TLR).

Figure: J774 macrophages were seeded overnight at 5 x 10 5 of viable cells/well. The next day the cells were pretreated with 20 µM Cytohalasin D for 1 h at 37ºC prior to addition of 5 µl of Zymosan particles. Phagocytosis was conducted for 2 hours and the amount of engulfed Zymosan was determined as described in the Assay Protocol. (I) Inhibition of phagocytosis. Panel A and B: images of non-treated cells. Panel C and D: treatment with Cytohalasin D. (II) Flow cytometry plot. Red line: untreated control cells; green line: macrophages with engulfed Zymosan particles; blue line: inhibition of phagocytosis by Cytochalasin D. (III) Zymosan Standard curve.

Key Information Description

Product SKU

BN00651

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 490/520 nm)

Species Reactivity

Mammalian

Applications

Rapid detection, quantification and validation of phagocytosis in convenient 96-well format Tracking ligand internalization and screening for effectors of phagocytosis

Features and Benefits

  • Simple procedure; takes ~ 3 hours
  • Fast and convenient
  • The assay is sensitive and stable
  • The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Kit Components

  • Phagocytosis Assay Buffer
  • Green Zymosan
  • 10X Quenching Solution

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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