Phosphoglucomutase Colorimetric Assay Kit (BN00987)

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SKU:
BN00987
  • Phosphoglucomutase Colorimetric Assay Kit (BN00987)
  • Phosphoglucomutase Colorimetric Assay Kit (BN00987)
€702

Description

ELISA Kit Technical ManualMSDS

Phosphoglucomutase Colorimetric Assay Kit

Phosphoglucomutase (PGM) plays a key role in carbohydrate metabolism and widely exists in all organisms. PGM interconverts Glucose-1-Phosphate (G1P) and Glucose-6-Phosphate (G6P) depending on the body requirement. When glycogen breaks down, G1P is generated and phosphoglucomutase converts G1P to G6P, which can go either to glycolytic pathway to generate ATP, or to pentose phosphate pathway to generate ribose and NADPH. On the other hand, when cells have extra energy, PGM converts G6P to G1P, which generates glycogen. In humans, phosphoglucomutase have 2 isoforms (PGM I and PGM II). PGM deficiency leads to glucose storage disease. Detection of abnormal phosphoglucomutase activity is crucial for diagnosis, prediction and treatment of the disease. In Assay Genie's phosphoglucomutase assay, PGM converts glucose-1-phosphate to glucose-6-phosphate; the glucose-6-phosphate is oxidized by glucose-6-phosphate dehydrogenase to form NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. The Phosphoglucomutase Assay Kit is simple, sensitive and rapid and can detect PGM activity even less than 1 mU/reaction.

Figure: (a).NADH standard curve (b) Phosphoglucomutase activity in pure PGM Positive Control, jurkat cell lysate (induced with 2 µM camptothecin) and bovine liver lysate respectively . Assays were performed following kit protocol.

Key Information Description

Product SKU

BN00987

Size

100 assays

Detection Method

Absorbance (450 nm)

Species Reactivity

Mammalian

Applications

The kit can detect phosphoglucomutase activity even less than 1 mU/reaction.

Features and Benefits

Sensitive assay for measuring phosphoglucomutase activity in various biological samples

Kit Components

  • PGM Assay Buffer
  • PGM Substrate (lyophilized)
  • PGM Enzyme Mix (Lyophilized)
  • PGM Developer (Lyophilized)
  • NADH Standard (Lyophilized)
  • PGM Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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