Plasma Kallikrein Activity Assay Kit (Colorimetric) (BN01154)

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BN01154
  • Plasma Kallikrein Activity Assay Kit (Colorimetric) (BN01154)
  • Plasma Kallikrein Activity Assay Kit (Colorimetric) (BN01154)
€708

Description

ELISA Kit Technical ManualMSDS

Plasma Kallikrein Activity Assay Kit (Colorimetric)

Plasma Prekallikrein (EC 3.4.21.34), is the glycosylated single chain zymogen precursor of the plasma serine protease Kallikrein. It circulates with kininogen and is activated by Factor XIIa to Kallikrein in the intrinsic coagulation pathway. Kallikrein activates plasminogen in fibrinolysis and cleaves kininogen in the bradykinin system of vasodilation. Prekallikrein deficiency is rare and causes increased activated partial thromboplastin time. Elevated plasma Prekallikrein is associated with diabetes and cardiovascular disease. Plasma Kallikrein inhibitors have been proposed as drugs to manage Hereditary Angioedema.

Assay Genie's Plasma Kallikrein Activity Assay Kit utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. The Plasma Kallikrein Specific Inhibitor (PKSI) selectively inhibits the ability of Plasma Kallikrein to the cleave the synthetic substrate. The kit is easy-to-use and can detect PK activity of Purified Plasma Kallikrein and Plasma Samples

Figure: (A) Kinetic progressive curves for different amounts of PK Enzyme and (B) Activated Plasma Samples are shown. (C) Standard curve for pNA (n = 3) was used to estimate (D) PK activity in Normal Pooled Human Plasma (n = 3) . Assays were performed according to the kit protocol

Key Info Description

Product Code

BN01154

Size

100 assays

Detection Method

Colorimetric (Absorbance 405 nm)

Species Reactivity

Mammalians

Applications

Measurement of Plasma Kallikrein activity in plasma samples and purified/crude enzyme preparations

Features & Benefits

  • Simple, rapid & convenient assay to measure Plasma Kallikrein
  • Includes Positive Control and Plasma Kallikrein and Plasma Kallikrein Inhibitors

Kit Components

  • PK Assay Buffer
  • PK Activator
  • PK Substrate
  • Human PK
  • PKSI Inhibitor
  • pNA Standard (0.1 M)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

Usage

For Research Use Only! Not For Use in Humans.

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