Preadipocyte Isolation Kit (BN00812)

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SKU:
BN00812
  • Preadipocyte Isolation Kit (BN00812)
  • Preadipocyte Isolation Kit (BN00812)
€613

Description

ELISA Kit Technical ManualMSDS

Preadipocyte Isolation Kit

Adipose tissue is loose connective tissue that accumulates in animals and serves primarily for energy storage, insulation, and thermoregulation. It is predominantly composed of adipocytes, but also contains a stromal vascular fraction consisting of preadipocytes, fibroblasts, vascular endothelial cells, and immune cells. Recent research has discovered a dynamic role of adipose tissue in the production and secretion of various hormones and as an active endocrine organ. The excessive accumulation of adipose is known as obesity. Ways to prevent over-accumulation of adipose is a field of intense research. In addition to obesity, adipose tissue has been found to participate in various physiological processes, including reproduction, angiogenesis, inflammation, cancer, and vascular homeostasis. Assay Genie's Preadipocyte Isolation Kit provides the reagents and tools to isolate the stromal vascular fraction from adipose tissue. The resulting stromal vascular fraction is cultured on a tissue-culture plate, where preadipocytes adhere. The preadipocytes retain the ability to proliferate and differentiate into adipocytes when treated with differentiation-inducing components (Cat. # K579, 3T3-L1 Differentiation Kit). The preadipocytes can be used to study the process of adipogenesis and the differentiated adipocytes can be used to study lipolysis, endocrine activity, cell-signaling, and metabolic dysfunction.

(a)

(b)

Figure: (a) Preadipocytes from mouse adipose tissue after 4 days of culturing. (b) Adipocytes 7 days after differentiation from preadipocytes using 3T3-L1 Differentiation Kit. Visible lipid droplets confirm the isolation of preadipocytes.

Key Information Description

Product SKU

BN00812

Size

For 5 g Tissue

Applications

Isolation of preadipocytes from adipose tissue

Features and Benefits

Simple, rapid & convenient method to isolate preadipocytes from adipose tissue.

Kit Components

  • Collagenase (0.2%)
  • Collagenase Stop Buffer
  • Adipocyte Wash Buffer
  • Adipocyte Lipolysis Buffer
  • Glycerol Assay Buffer
  • Glycerol Probe (in DMSO, anhydrous)
  • Glycerol Enzyme Mix (Lyophilized)
  • Glycerol Standard (100 mM)
  • Isoproterenol (10 mM)
  • Cell strainer (100 µm)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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