The Proteasome 20S LMP7 Polyclonal Antibody is a valuable tool for researchers studying the proteasome complex and its role in protein degradation. This antibody, raised in rabbits, is highly specific for the LMP7 subunit of the 20S proteasome and is validated for use in various applications such as Western blotting and immunohistochemistry.The 20S proteasome is a key player in the regulation of protein turnover within cells, with the LMP7 subunit specifically involved in antigen processing and presentation. By targeting LMP7 with this polyclonal antibody, researchers can investigate the function of the proteasome complex in processes such as immune surveillance, cell cycle regulation, and apoptosis.
Studies have shown that dysregulation of the proteasome complex, including alterations in LMP7 expression, is associated with various diseases such as cancer, neurodegenerative disorders, and autoimmune conditions. By utilizing the Proteasome 20S LMP7 Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic targets for intervention.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
Purification Method:
Affinity purification
Gene ID:
5696
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Proteasome 20S LMP7 antibody (CAB22542) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
