The PTPN1 Polyclonal Antibody is a valuable tool for researchers studying the PTPN1 protein, also known as protein tyrosine phosphatase non-receptor type 1. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.PTPN1 plays a crucial role in the regulation of various signaling pathways involved in cell growth, differentiation, and immune response. By binding to PTPN1, this antibody enables the detection and analysis of the protein in a wide range of cell types, making it an ideal choice for studies in cell biology, signal transduction, and cancer research.
Understanding the function of PTPN1 is essential for unraveling its involvement in disease processes, including cancer development, immune system dysregulation, and metabolic disorders. By using the PTPN1 Polyclonal Antibody, researchers can gain valuable insights into the mechanisms underlying these diseases and identify potential therapeutic targets for intervention.
Product Name:
PTPN1 Polyclonal Antibody
SKU:
CAB22243
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 281-435 of human PTPN1 (NP_002818.1).
The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5770
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using [KO Validated] PTPN1 Rabbit pAb (CAB22243) at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
