Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) (BN00980)

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BN00980
  • Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) (BN00980)
  • Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) (BN00980)
  • Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) (BN00980)
€864

Description

ELISA Kit Technical ManualMSDS

Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)

Purine Nucleoside Phosphorylase (PNP) (E.C. 2.4.2.1.) is an enzyme involved in purine metabolism and it catalyzes the cleavage of the glycosidic bond of ribo- or deoxyribonucleosides, in the presence of inorganic phosphate as a second substrate, to generate the purine base and ribose-1-phosphate or deoxyribose-1-phosphate. It is one of the enzymes of the nucleotide salvage pathways that allows the cell to produce nucleotide monophosphates when the de novo synthesis pathway has been interrupted or is non-existent (as is the case in the brain). PNP is a cytosolic enzyme. PNP deficiency disease leads to toxic buildup of deoxyguanosine in T-cells leading to T-lymphocytopenia with no apparent effects on B-lymphocyte function. Inhibition of PNP is an important target for chemotherapeutic applications and treatment of T- cell mediated autoimmune diseases. PNP deficiency is also associated with neurological problems. In Assay Genie's Purine Nucleoside Phosphorylase Activity Assay, hypoxanthine formed from the breakdown of inosine is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the PNP Probe. The fluorescent product is measured at Ex/Em = 535/587 nm. Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase.

Figure: (a) Hypoxanthine Standard Curve, (b) Purine Nucleoside Phosphorylase Activity in Jurkat Cell (T-lymphocyte) lysate (315 ng) and Positive Control; BG: background (c) PNP specific activity in Jurkat Cell lysate. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00980

Size

100 assays

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Applications

Detection of Purine Nucleoside Phosphorylase activity in variety of samples

Features and Benefits

  • Rapid, simple & convenient
  • Limit of quantification is 0.005 µU recombinant Purine Nucleoside Phosphorylase

Kit Components

  • PNP Assay Buffer (10x)
  • Enzyme Mix
  • Inosine Substrate
  • PNP Probe (in dry DMSO)
  • Hypoxanthine Standard (10 mM)
  • PNP Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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