Recombinant Human ALDOA Protein (His Tag) (RPES4352)



ELISA Kit Technical Manual

Recombinant Human ALDOA Protein (His Tag)

Background:Fructose Bisphosphate Aldolase A (ALDOA) belongs to the class I fructose-bisphosphate aldolase family. ALDOA is a glycolytic enzyme that catalyzes the reversible conversion of fructose,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, Aldolase A in muscle, Aldolase B in liver and aldolase C in brain. Aldolase A Interacts with SNX9 and WAS. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. In addition, Aldolase A plays an important role in glycolysis and gluconeogenesis; it may also act as a scaffolding protein.
Name:Recombinant Human ALDOA Protein (His Tag)
Assay Genie SKU:RPES4352
Synonyms:Fructose-Bisphosphate Aldolase A; Lung Cancer Antigen NY-LU; Muscle-Type Aldolase; ALDOA; ALDA
Expression host:E.coli
Mol Mass:40.5 kDa
AP Mol Mass:36 kDa
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping:This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Formulation:Supplied as a 0.2 um filtered solution of 20mM TrisHCl, 100mM NaCl, 20% Glycerol, pH 8.0.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate -> glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC; Carbohydrate Metabolism - fructose and mannose; Lyase; Carbohydrate Metabolism - pentose phosphate pathway; Mitochondrial

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: I band; extracellular space; membrane; extracellular region; M band; cytosol; nucleus; actin cytoskeleton

Molecular Function:tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding

Biological Process: platelet activation; striated muscle contraction; glycolysis; glucose metabolic process; actin filament organization; pathogenesis; protein homotetramerization; gluconeogenesis; muscle maintenance; regulation of cell shape; fructose 1,6-bisphosphate metabolic process; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process

Disease: Glycogen Storage Disease Xii

NCBI Summary:The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]
UniProt Code:P04075
NCBI GenInfo Identifier:113606
NCBI Gene ID:226
NCBI Accession:P04075.2
UniProt Secondary Accession:P04075,Q6FH76, Q6FI10, Q96B15, Q9BWD9, Q9UCN2, B4DXI7
UniProt Related Accession:P04075
Molecular Weight:364
NCBI Full Name:Fructose-bisphosphate aldolase A
NCBI Synonym Full Names:aldolase A, fructose-bisphosphate
NCBI Official Symbol:ALDOA
NCBI Official Synonym Symbols:ALDA; GSD12; HEL-S-87p
NCBI Protein Information:fructose-bisphosphate aldolase A; muscle-type aldolase; lung cancer antigen NY-LU; fructose,6-bisphosphate triosephosphate-lyase; epididymis secretory sperm binding protein Li 87p
UniProt Protein Name:Fructose-bisphosphate aldolase A
UniProt Synonym Protein Names:Lung cancer antigen NY-LU; Muscle-type aldolase
Protein Family:Fructose-bisphosphate aldolase
UniProt Gene Name:ALDOA
UniProt Entry Name:ALDOA_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
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