Human Galectin-8/LGALS8 Recombinant Protein (RPES4696)

Product type:
Recombinant Protein
Host species:


ELISA Kit Technical Manual

Recombinant Human Galectin-8/LGALS8 Protein (Active)

Background:The Galectin family of proteins, with specificity for Nacetyllactosaminecontaining glycoproteins, consists of beta-galactoside binding lectins containing homologous carbohydrate recognition domains (CRDs). They also possess hemagglutination activity, which is attributable to their bivalent carbohydrate binding properties. Galectins are active both intracellularly and extracellularly. Although they are localized primarily in the cytoplasm and lack a classical signal peptide, galectins can also be secreted by one or more unidentified, non-classical, secretory pathways. They have diverse effects on many cellular functions including adhesion, migration, polarity, chemotaxis, proliferation, apoptosis, and differentiation. Galectins may therefore play a key role in many pathological states, including autoimmune diseases, allergic reactions, inflammation, tumor cell metastasis, atherosclerosis, and diabetic complications. The galectins have been classified into the prototype galectins(1, 2, 5, 7, 10, 11, 13, 14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins(Galectin3) containing one CRD linked to a nonlectin domain, and the tandemrepeat Galectins(4, 6, 8, 9, 12) consisting of two CRDs joined by a linker peptide.Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified nonclassical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell surface glycoproteins.
Name:Recombinant Human Galectin-8/LGALS8 Protein (Active)
Assay Genie SKU:RPES4696
Synonyms:Galectin-8; Gal-8; Po66 Carbohydrate-Binding Protein; Po66-CBP; Prostate Carcinoma Tumor Antigen 1; PCTA; LGALS8
Expression host:E.coli
Sequence:Met 1-Trp317
Mol Mass:35.8 kDa
AP Mol Mass:32 kDa
Bio Activity:Measured by its ability to agglutinate human red blood cells.
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 250mM NaCl, pH 7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:galectin-8: 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell adhesion

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytosol; extracellular space; membrane

Molecular Function:carbohydrate binding; protein binding

NCBI Summary:This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:O00214
NCBI GenInfo Identifier:292495094
NCBI Gene ID:3964
NCBI Accession:O00214.4
UniProt Secondary Accession:O00214,O15215, Q5T3P5, Q5T3Q4, Q8TEV1, Q96B92, Q9BXC8 Q9H584, Q9H585, Q9UEZ6, Q9UP32, Q9UP33,
UniProt Related Accession:O00214
Molecular Weight:40,397 Da
NCBI Full Name:Galectin-8
NCBI Synonym Full Names:galectin 8
NCBI Official Symbol:LGALS8
NCBI Official Synonym Symbols:Gal-8; PCTA1; PCTA; Po66-CBP
NCBI Protein Information:galectin-8
UniProt Protein Name:Galectin-8
UniProt Synonym Protein Names:Po66 carbohydrate-binding protein; Po66-CBP; Prostate carcinoma tumor antigen 1; PCTA
Protein Family:Galectin
UniProt Gene Name:LGALS8
UniProt Entry Name:LEG8_HUMAN
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