Description
| Product Name: | Recombinant Human Pro-neuregulin-1/NRG1 (Beta1) Protein |
| SKU: | RPCB1618 |
| Size: | 10µg |
| Tag: | No-Tag |
| Reactivity: | Human |
| Expression Host: | E. coli |
| Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant Human Pro-neuregulin-1/NRG1 (Beta1) Protein , tested reactivity inE. coliand has been validated in SDS-PAGE.100% guaranteed. |
| Endotoxin: | < 1EU/μg |
| Purity: | > 92% by SDS-PAGE. |
| Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
| Gene ID: | 3084 |
neuregulin-1 (heregulin-1， NRG1) is a member of neuregulin family, which is comprised of four genes thatencode a large number of secreted or membrane-bound isoforms. All family members share an EGF-likedomain that interacts with the ErbB family of tyrosine kinase receptors. NRG1 isoforms can be classified intotype I, type II and type III isoforms. NRG1 directs ligand for ERBB3 and ERBB4 tyrosine kinase receptors,concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylationand activation of the ERBB receptors. NRG proteins show distinct spatial and temporal expression patterns andplay important roles during development of both the nervous system and the heart.
| Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
| Reconstitution: | Centrifμge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
| Swiss-Prot: | Q02297-6（Beta1） |
 | Recombinant Human Pro- neuregulin-1/NRG1（Beta1） Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 10-15 kDa. |
 | Recombinant Human Pro- neuregulin-1/NRG1 (Beta1) stimulates serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is typically 0.62-2.48 ng/mL, corresponding to a specific activity of 4.03×105~1.61×106 units/mg. |
