The RYR1 Polyclonal Antibody is a valuable tool for researchers studying the ryanodine receptor 1 protein, a crucial component in muscle contraction and calcium release. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the RYR1 protein, researchers can analyze and detect its presence in various cell types, making it ideal for investigations in muscle physiology, neurology, and pharmacology.Ryanodine receptor 1 is a critical calcium channel involved in excitation-contraction coupling in skeletal muscle cells, playing a key role in muscle function and regulation.
Dysregulation of RYR1 has been associated with muscle disorders like malignant hyperthermia and central core disease, making it a significant target for research in muscle-related pathologies. By understanding the function and regulation of RYR1, researchers can develop targeted therapies for muscle diseases and improve overall muscle health.
Product Name:
RYR1 Polyclonal Antibody
SKU:
CAB23056
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human RYR1 (NP_000531.2).
This gene encodes a ryanodine receptor found in skeletal muscle. The encoded protein functions as a calcium release channel in the sarcoplasmic reticulum but also serves to connect the sarcoplasmic reticulum and transverse tubule. Mutations in this gene are associated with malignant hyperthermia susceptibility, central core disease, and minicore myopathy with external ophthalmoplegia. Alternatively spliced transcripts encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
6261
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of Mouse skeletal muscle, using RYR1 antibody (CAB23056) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
