Senescence Detection Kit (BN00587)

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SKU:
BN00587
  • Senescence Detection Kit (BN00587)
  • Senescence Detection Kit (BN00587)
€541

Description

ELISA Kit Technical ManualMSDS

Senescence Detection Kit

Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of Beta-galactosidase (SA-Beta-Gal) activity, and altered patterns of gene expression. The Senescence detection kit is designed to histochemically detect SA-Beta-Gal activity in cultured cells and tissue sections, a known characteristic of senescent cells. The SA-Beta-Gal is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.

Figure: Hydrogen peroxide induced senescence in HeLa cells. 1X10 4 HeLa cells were seeded in 24-well plate with (B) or without 10 µM H 2 O 2 treatment (A) for 6 days. All cells were fixed in Fixative Solution and stained overnight according to the kit protocol.

Key Information Description

Product SKU

BN00587

Size

250 Stainings

Species Reactivity

Mammalian

Applications

The Senescence Detection Kit is designed to histochemically detect specific senescence marker in distinct pH in cultured cells and tissue sections.

Features and Benefits

  • Simple procedure; takes around than 30 minutes
  • Fast and convenient
  • The specific histochemical marker is only present in senescent cells and is not found in presenescent, quiescent or immortal cells.

Kit Components

  • Fixative Solution (1X)
  • X-Gal (150 mg, lyophilized)
  • Staining Solution (1X)
  • Staining Supplement (100X)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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