Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of Beta-galactosidase (SA-Beta-Gal) activity, and altered patterns of gene expression. The Senescence detection kit is designed to histochemically detect SA-Beta-Gal activity in cultured cells and tissue sections, a known characteristic of senescent cells. The SA-Beta-Gal is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.

Figure: Hydrogen peroxide induced senescence in HeLa cells. 1X10 4 HeLa cells were seeded in 24-well plate with (B) or without 10 µM H 2 O 2 treatment (A) for 6 days. All cells were fixed in Fixative Solution and stained overnight according to the kit protocol.