Sphingomyelinase Activity Colorimetric Assay Kit (BN00826)

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SKU:
BN00826
  • Sphingomyelinase Activity Colorimetric Assay Kit (BN00826)
  • Sphingomyelinase Activity Colorimetric Assay Kit (BN00826)
  • Sphingomyelinase Activity Colorimetric Assay Kit (BN00826)
€517

Description

ELISA Kit Technical ManualMSDS

Sphingomyelinase Activity Colorimetric Assay Kit

Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation, and sterol homeostasis. There are five distinct types of SMases. Assay Genie's SMase Activity Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using colorimetry (OD 570 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce an intermediate that reacts with a highly specific probe to generate color. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 1.25 mU/ml (~5 µU/mg tissue/cells) in a variety of samples.

Figure: (a) Choline Standard Curve, (b) SMase activity of brain lysate (4.5 mg), Positive Control (1 µl) (c) SMase specific activity in brain lysate. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00826

Size

100 assays

Detection Method

Absorbance (OD 570 nm)

Applications

  • Measurement of SMase activity in various tissues/cells extracts
  • Screening of SMase inhibitors

Features and Benefits

  • Simple, rapid & convenient
  • The assay can detect sphingomyleinase activity as low as 1.25 mU/ml (~5 µU/mg tissue/cells) in a variety of samples

Kit Components

  • SMase Assay Buffer
  • SMase Probe (DMSO)
  • SMase Substrate (Lyophilized)
  • SMase Enzyme Mix I (Lyophilized)
  • SMase Enzyme Mix II (Lyophilized)
  • Choline Standard (Lyophilized)
  • SMase Positive Control (Lyophilized)
  • SMase Extraction Detergent

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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