Sphingomyelinase Activity Fluorometric Assay Kit (BN00803)

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SKU:
BN00803
  • Sphingomyelinase Activity Fluorometric Assay Kit (BN00803)
  • Sphingomyelinase Activity Fluorometric Assay Kit (BN00803)
  • Sphingomyelinase Activity Fluorometric Assay Kit (BN00803)
€529

Description

ELISA Kit Technical ManualMSDS

Sphingomyelinase Activity Fluorometric Assay Kit

Sphingomyelinase (SMase) cleaves sphingomyelin to produce phosphocholine and ceramide. The activation of SMase leads to increased production of ceramide, which acts as a lipid second messenger that induces a variety of cell regulatory phenomena such as apoptosis, cell differentiation, cell proliferation and sterol homeostasis. Assay Genie's SMase Fluorometric Assay Kit provides a simple and sensitive method for measuring neutral-SMase enzymatic activity using fluorescence (Ex/Em = 535/587 nm). Neutral sphingomyelinase (N-SMase) is a Mg2+ sensitive enzyme that can be activated by a host of physiologically relevant and structurally diverse molecules. In this assay, SMase converts sphingomyelin substrate to phosphocholine and ceramide, which is then further utilized to produce fluorescence. This high-throughput adaptable assay kit can detect sphingomyelinase activity as low as 10 µU/ml in a variety of samples.

Figure: (a) Choline Standard Curve, (b) SMase activity of brain lysate (0.15 mg), Positive Control (5 µl) and (c) SMase specific activity in rat brain lysate. Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00803

Size

100 assays

Detection Method

Fluorescence (Ex/Em 535/587 nm)

Application

Measurement of SMase activity in various tissues/cells extracts

Features and Benefits

  • Simple, rapid & convenient
  • The assay can detect sphingomyleinase activity as low as 10 µU/ml in a variety of samples.

Kit Components

  • SMase Assay Buffer
  • SMase Probe (DMSO)
  • SMase Substrate (Lyophilized)
  • SMase Enzyme Mix I (Lyophilized)
  • SMase Enzyme Mix II (Lyophilized)
  • Choline Standard (Lyophilized)
  • SMase Positive Control (Lyophilized)
  • SMase Extraction Detergent

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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