The Strada Polyclonal Antibody is a crucial tool for researchers studying the protein Strada, which is involved in pathways related to cell growth and proliferation. This rabbit-derived antibody is highly specific for human samples and is optimized for Western blot applications. By targeting the Strada protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it a valuable asset for research in cancer biology and signal transduction mechanisms.Strada, also known as Ste20-related kinase adaptor alpha, is a key player in the regulation of cell cycle progression and survival signaling.
Its role in promoting cell growth and suppressing apoptosis makes it a potential target for investigating diseases such as cancer and neurodegenerative disorders. By understanding the function of Strada, researchers can uncover potential therapeutic strategies for targeting aberrant cell signaling pathways implicated in various pathological conditions.
Product Name:
STRADA Polyclonal Antibody
SKU:
CAB21707
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human STRADA (NP_699166.2).
LYK5; PMSE; Stlk; STRAD; NY-BR-96; STRAD alpha; STRADA
Positive Sample:
Mouse skeletal muscle
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
48kDa
Observed MW:
Refer to figures
The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known.
Purification Method:
Affinity purification
Gene ID:
92335
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse skeletal muscle, using STRADA Rabbit pAb (CAB21707) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
