Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit (BN00601)

(No reviews yet) Write a Review
SKU:
BN00601
  • Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit (BN00601)
  • Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit (BN00601)
€511

Description

ELISA Kit Technical ManualMSDS

Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit

Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. It catalyzes the dismutation of the superoxide anion into hydrogen peroxide and molecular oxygen. The sensitive SOD assay kit utilizes WST-1 that produces a water-soluble formazan dye upon reduction with superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD (below). Therefore, the inhibition activity of SOD can be determined by a colorimetric method.

Figure: SOD Activity (% inhibition rate): human serum (10 µl) and isolated mitochondria from Jurkat cells (10 µg), and yeast (Saccromyces cerevisiae, 100 ug), was used to determine SOD Activity according to the kit protocol. Activity was measured in 15 min. at 37°C.

Key Information Description

Product SKU

BN00601

Size

100 assays

Detection Method

Absorbance (450 nm)

Applications

Kit contains the necessary reagents for convenient measurement of activity of Superoxide Dismutase (SOD) by colorimetric method.

Features and Benefits

  • Simple one-step procedure; takes around than 30 minutes
  • Fast and convenient

Kit Components

  • WST Solution
  • SOD Enzyme Solution
  • SOD Assay Buffer
  • SOD Dilution Buffer

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

View AllClose