Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit (BN00894)

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SKU:
BN00894
  • Ceruloplasmin Activity Colorimetric Assay Kit (BN00893)
  • Ceruloplasmin Activity Colorimetric Assay Kit (BN00893)
  • Ceruloplasmin Activity Colorimetric Assay Kit (BN00893)
€744

Description

ELISA Kit Technical ManualMSDS

Triose Phosphate Isomerase (TPI) Activity Colorimetric Assay Kit

Triose Phosphate Isomerase (TPI or TIM: EC 5.3.1.1) is an important enzyme for glycolysis. It reversibly interconverts dihydroxyacetone phosphate and glyceraldehyde-3-phosphate, thus maintaining the equilibrium of these two triose phosphates. TPI connects glycolysis to pentose phosphate pathway and lipid metabolism. It is a stable homodimer found in almost all organisms. In humans, TPI deficiency is a rare multisystem disorder and leads to progressive neurological dysfunction, characterized by hemolytic anemia, cardiomyopathy and progressive neuromuscular impairment. Assay Genie's Triose Phosphate Isomerase Activity Assay kit provides a quick and easy way for monitoring Triose Phosphate Isomerase activity in a variety of samples. In this kit, Triose Phosphate Isomerase converts dihydroxyacetone phosphate into glyceradehyde-3-phosphate, which reacts with the Enzyme Mix & Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive, & high-throughput and can detect Triose Phosphate Isomerase activity as low as 40 mU/ml.

Figure: (a) NADH Standard Curve. (b) Kinetic measurement of Triose Phosphate Isomerase (TPI) activity in various samples. (c) Relative TPI activity was calculated in lysates prepared from rat muscle (0.5 µg), MCF-7 cells (0.8 µg), and HeLa cells (0.84 µg). Assays were performed following the kit protocol.

Key Information Description

Product SKU

BN00894

Size

100 assays

Detection Method

Absorbance (OD 450 nm)

Applications

  • Measurement of TPI activity in various tissues and cells.
  • Analysis of glycolysis & pentose phosphate pathway and lipid metabolism

Features and Benefits

  • Simple, highly sensitive, and high-throughput.
  • The assay can measure Triose Phosphate Isomerase activity as low as 40 mU/ml.

Kit Components

  • TPI Assay Buffer
  • TPI Substrate (Lyophilized)
  • TPI Enzyme Mix (Lyophilized)
  • TPI Developer (Lyophilized)
  • NADH Standard (Lyophilized)
  • TPI Positive Control (Lyophilized)

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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