Tyrosine Aminotransferase Activity Assay Kit (Fluorometric) (BN00722)

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SKU:
BN00722
  • Tyrosine Aminotransferase Activity Assay Kit (Fluorometric) (BN00722)
  • Tyrosine Aminotransferase Activity Assay Kit (Fluorometric) (BN00722)
  • Tyrosine Aminotransferase Activity Assay Kit (Fluorometric) (BN00722)
€649

Description

ELISA Kit Technical ManualMSDS

Tyrosine Aminotransferase Activity Assay Kit (Fluorometric)

Tyrosine Aminotransferase (EC 2.6.1.5), also known as Tyrosine Transaminase (TAT), is a mitochondrial enzyme encoded by the TAT gene in the nuclear DNA. It catalyzes the transamination of tyrosine by transferring an amine group to an ketoglutarate molecule. Tyrosine Aminotransferase is highly expressed in the liver and its deficiency, usually inherited, leads to a buildup of toxic levels of tyrosine in the body called tyrosinemia. Tyrosinemia causes skin and corneal lesions, kidney and liver disorders, and could lead to mental retardation in newborns. Assay Genie's Tyrosine Aminotransferase Activity Assay Kit is a simple plate-based fluorometric assay for the measurement of TAT activity in biological samples. The transamination of tyrosine by TAT produces glutamate (through the transfer of amine group to ketoglutarate), which converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. The assay can detect as low as 4 µU of tyrosine aminotransferase.

(a)

(b)

(c)

Figure: (a) Glutamate standard curve, (b) Enzyme kinetics for rat liver lysate (4.4 µg protein per well) and Jurkat cell lysate (10 µg protein per well), (c) TAT activity in rat liver tissue lysate and Jurkat cell lysate.

Key Information Description

Product SKU

BN00722

Size

100 assays

Kit Summary

  • Detection method- Fluorescence (Ex/Em 535/587 nm)
  • Species reactivity- Mammalian
  • Applications- The kit detects TAT activity with a detection limit of 4 µU per well.

Detection Method

Fluorescence (Ex/Em = 535/587 nm)

Species Reactivity

Eukaryotes

Applications

Measurement of TAT activity in cell and tissue lysates using a 96-well plate format

Features and Benefits

  • Simple procedure
  • Fast and convenient
  • Sensitive assay for measuring TAT activity in various biological samples, high-throughput compatible

Kit Components

  • TAT Assay Buffer
  • TAT Substrate I
  • TAT Substrate II
  • TAT Developer
  • TAT Enzyme Mix
  • TAT Probe
  • Glutamate Standard (0.1 M)
  • TAT Positive Control

Storage Conditions

-20°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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Additional Information

Product type:
Assay
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