The UBE3B Polyclonal Antibody (PACO05660) is a valuable tool for researchers studying UBE3B, a critical protein involved in protein degradation and cellular processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for various applications including Western blotting.UBE3B is an essential component of the ubiquitin-proteasome system, playing a role in the targeted degradation of proteins within the cell.
Dysregulation of UBE3B has been linked to various diseases, including cancer and neurodegenerative disorders, making it a promising target for therapeutic interventions. By utilizing the UBE3B Polyclonal Antibody, researchers can effectively investigate the expression and function of UBE3B in different cell types and disease contexts, providing valuable insights into its potential role in disease pathology and therapeutic targeting.
Antibody Name:
UBE3B Antibody (PACO05660)
Antibody SKU:
PACO05660
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
ELISA:1:10000, WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from the Internal region of human UBE3B.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
UBE3B; Ubiquitin-protein ligase E3B
UniProt Protein Function:
UBE3B: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. 3 isoforms of the human protein are produced by alternative splicing.Protein type: Ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating systemChromosomal Location of Human Ortholog: 12q24.11Molecular Function: ligase activity; ubiquitin-protein ligase activityBiological Process: protein ubiquitination during ubiquitin-dependent protein catabolic processDisease: Kaufman Oculocerebrofacial Syndrome
UniProt Protein Details:
NCBI Summary:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012]
