Anti-SARS-CoV-2 Spike Antibody, S1 Monoclonal (CAB20022)



Technical Manual

SARS-CoV-2 spike antibody, S1 Monoclonal (CAB20022)

Product Name: SARS-CoV-2 spike antibody, S1 Monoclonal
Product SKU: CAB20022
Size: 50μL, 100μL
Host Species: Rabbit
Purification Method: Affinity purification
Isotype: IgG

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Immunogen: Recombinant fusion protein of 2019-nCoV Spike S1.

Email for sequence.

Gene ID: 43740568
Swiss Prot: P0DTC2
Calculated MW:  
Observed MW:  
Reactivity: SARS-CoV-2
Application: ELISA, FC, IHC, IF, IP
Recommended Dilution: ELISA 1:1000 - 1:5000
FC 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage Buffer: Store at 4°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key: Flow cytometry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Positive Samples:  
Cellular Location: Accumulates in the endoplasmic reticulum-Golgi intermediate compartment, where it participates in virus particle assembly. Colocalizes with S in the host endoplasmic reticulum-Golgi intermediate compartment. Some S oligomers are transported to the host plasma membrane, where they may mediate cell-cell fusion (By similarity).
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Additional Information

Product type:
Host Species:
Antibody Type:
Monoclonal Antibody
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