Caveolin-1 (Phospho-Tyr14) Fluorometric Cell-Based ELISA Kit

SKU:
FBCAB00061
Product type:
ELISA Kit
ELISA Type:
Cell Based
€599

Description

Technical Manual

Caveolin-1 (Phospho-Tyr14) Cell-Based ELISA Kit

The Caveolin-1 (Phospho-Tyr14) Cell-Based ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can monitor Caveolin-1 phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Caveolin-1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caveolin-1 phosphorylation.

How does our Caveolin-1 (Phospho-Tyr14) Fluorometric Cell-Based ELISA Kit work?

Qualitative determination of Caveolin-1 (Phospho-Tyr14) concentration is achieved by an indirect ELISA format. In essence, Caveolin-1 (Phospho-Tyr14) is captured by Caveolin-1 (Phospho-Tyr14)-specific primary (1°) antibodies while Dye 1-conjugated and Dye 2-conjugated secondary (2°) antibodies bind the Fc region of the 1° antibody. Through this binding, the dye conjugated to the 2° antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values.
2. An antibody against the nonphosphorylated counterpart of Caveolin-1 (Phospho-Tyr14) is also provided for normalization purposes. The RFU values obtained for non-phosphorylated Caveolin-1 can be used to normalize the RFU value for phosphorylated Caveolin-1.

Caveolin-1 (Phospho-Tyr14) Fluorometric Cell-Based ELISA Kit -Information

Product Name:Caveolin-1 (Phospho-Tyr14) Fluorometric Cell-Based ELISA Kit
Product Code/SKU:FBCAB00061
Description:The Caveolin-1 (Phospho-Tyr14) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Caveolin-1 (Phospho-Tyr14) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Caveolin-1 (Phospho-Tyr14) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
Dynamic Range:> 5000 Cells
Detection Method:Fluorometric
Storage/Stability:4°C/6 Months
Reactivity:Human, Mouse, Rat
Assay Type:Cell-Based ELISA
Database Links:Gene ID: 857, UniProt ID: Q03135, OMIM #: 601047/612526, Unigene #: Hs.74034
Format:Two 96-Well Plates
NCBI Gene Symbol:CAV1
Sub Type:Phospho
Target Name:Phospho-Caveolin-1 (Tyr14)

Kit Principle

Figure: Schematic representation of Assay Genie Cell-Based Fluorometric ELISA principle

Kit components

Quantity

96-Well Black Cell CultureClear-Bottom Microplate2 plates
10X TBS24 ml
Quenching Buffer24 ml
Blocking Buffer50 ml
15X Wash Buffer50 ml
Primary Antibody Diluent12 ml
100x Anti-Phospho Target Antibody 60 µl
100x Anti-Target Antibody60 µl
Anti-GAPDH Antibody110 µl
Dye-1 Conjugated Anti-Rabbit IgG Antibody6 ml
Dye-2 Conjugated Anti-Mouse IgG Antibody6 ml
Adhesive Plate Seals2 seals

Additional equipment and materials required

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Fluorescent plate reader with two channels at Ex/Em: 651/667 and 495/521
  • Micropipettes capable of measuring volumes from 1 µl to 1 ml
  • Deionized or sterile water (ddH2O)
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)

Kit Protocol

This is a summarized version of the kit protocol. Please view the technical manual of this kit for information on sample preparation, reagent preparation and plate lay out.

1.Seed 200 µl of desired cell concentration in culture medium into each well of the 96-well plates. For suspension cells and loosely attached cells, coat the plates with 100 µl of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µl of 1x TBS, twice.
5.Fix the cells by incubating with 100 µl of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µl 1x Wash Buffer for 3 minutes. The plate can be stored at 4°C for a week.
7.Add 100 µl of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 3 minutes each time.
9.Dispense 200 µl of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µl of 1x Wash Buffer for 3 minutes each time.
11.Add 50 µl of Primary Antibody Mixture P to corresponding wells for Caveolin-1 (Phospho-Tyr14) detection. Add 50 µl of Primary Antibody Mixture NP to the corresponding wells for total Caveolin-1 detection. Cover the plate with parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µl of 1x Wash Buffer for 3 minutes each time.
13.Add 50 ul of Secondary Antibody Mixture to corresponding wells and incubate for 1.5 hours at room temperature in the dark.
14.Wash 3 times with 200 µl of 1x Wash Buffer for 3 minutes each time.
15.Read the plate(s) at Ex/Em: 651/667 (Dye 1) and 495/521 (Dye 2). Shield plates from direct light exposure.
16.Wash 3 times with 200 µl of 1x Wash Buffer for 5 minutes each time.

Caveolin-1 (Phospho-Tyr14) - Protein Information

UniProt Protein Function:Caveolin-1: May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)- mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3- dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Homooligomer. Interacts with GLIPR2, NOSTRIN, SNAP25 and syntaxin. Interacts with rotavirus A NSP4. Interacts (via the N- terminus) with DPP4; the interaction is direct. Interacts with CTNNB1, CDH1 and JUP. Interacts with BMX and BTK. Expressed in muscle and lung, less so in liver, brain and kidney. Belongs to the caveolin family. 2 isoforms of the human protein are produced by alternative initiation.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 7q31.1

Cellular Component: protein complex; focal adhesion; integral to plasma membrane; basolateral plasma membrane; endoplasmic reticulum; lipid particle; cell cortex; caveola; acrosomal membrane; cilium; lipid raft; Golgi membrane; early endosome membrane; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; intracellular; cytoplasmic vesicle; endosome

Molecular Function:identical protein binding; protein binding; protease activator activity; enzyme binding; cholesterol binding; patched binding; protein complex scaffold; structural molecule activity; nitric-oxide synthase binding; protein kinase binding; receptor binding

Biological Process: mammary gland involution; viral reproduction; negative regulation of epithelial cell differentiation; negative regulation of tyrosine phosphorylation of Stat5 protein; nitric oxide homeostasis; negative regulation of BMP signaling pathway; calcium ion homeostasis; protein localization; sequestering of lipid; regulation of the force of heart contraction by chemical signal; regulation of fatty acid metabolic process; negative regulation of protein binding; inactivation of MAPK activity; regulation of smooth muscle contraction; maintenance of cellular protein localization; skeletal muscle development; cytosolic calcium ion homeostasis; negative regulation of nitric-oxide synthase activity; cellular response to starvation; membrane depolarization; cholesterol homeostasis; response to estrogen stimulus; T cell costimulation; negative regulation of endothelial cell proliferation; negative regulation of protein ubiquitination; regulation of nitric-oxide synthase activity; response to calcium ion; response to progesterone stimulus; negative regulation of JAK-STAT cascade; leukocyte migration; lactation; vesicle organization and biogenesis; negative regulation of peptidyl-serine phosphorylation; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; nitric oxide metabolic process; mammary gland development; calcium ion transport; negative regulation of cytokine and chemokine mediated signaling pathway; angiogenesis; vasculogenesis; protein homooligomerization; vasoconstriction; cholesterol transport; negative regulation of MAPKKK cascade; negative regulation of nitric oxide biosynthetic process; MAPKKK cascade; positive regulation of metalloenzyme activity; positive regulation of peptidyl-serine phosphorylation; regulation of peptidase activity; cellular calcium ion homeostasis; triacylglycerol metabolic process; regulation of blood coagulation; response to hypoxia; positive regulation of vasoconstriction; blood coagulation; vascular endothelial growth factor receptor signaling pathway

Disease: Lipodystrophy, Congenital Generalized, Type 3; Partial Lipodystrophy, Congenital Cataracts, And Neurodegeneration Syndrome; Pulmonary Hypertension, Primary, 3

NCBI Summary:The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]
UniProt Code:Q03135
NCBI GenInfo Identifier:13637934
NCBI Gene ID:857
NCBI Accession:Q03135.4
UniProt Secondary Accession:Q03135,Q9UGP1, Q9UNG1, Q9UQH6,
UniProt Related Accession:Q03135
Molecular Weight:178
NCBI Full Name:Caveolin-1
NCBI Synonym Full Names:caveolin 1, caveolae protein, 22kDa
NCBI Official Symbol:CAV1  
NCBI Official Synonym Symbols:CGL3; PPH3; BSCL3; LCCNS; VIP21; MSTP085  
NCBI Protein Information:caveolin-1; cell growth-inhibiting protein 32
UniProt Protein Name:Caveolin-1
Protein Family:Caveolin
UniProt Gene Name:CAV1  
UniProt Entry Name:CAV1_HUMAN