COVID-19 Human IgA PharmaGenie ELISA Kit (SBRS1716)

Product type:


COVID-19 Human IgA PharmaGenie ELISA Kit - Kit Data

Product SKU:SBRS1716
Gene Names:IGA
Target Species:Human
Compatible Sample Types:Dried Blood, Plasma, Serum
Design Principle:Indirect
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative / Semi-Quantitative
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C.

Kit Components:

1.SARS-CoV-2 N-protein coated 96-well microplate
2.Wash Buffer
3.Positive Control
4.Negative Control
5.HRP-anti positive control IgA Antibody
6.HRP-anti Human IgA Antibody
7.TMB One-Step Substrate
8.Stop Solution
9.Assay Diluent

Other materials and equipment required:

The Assay Genie COVID-19 Human IgA ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Microplate reader capable of measuring absorbance at 450 nm
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • 100 ml and 1 liter graduated cylinders
  • Absorbent paper
  • Distilled or deionized water

Kit Protocol:

1.Prepare all reagents, samples and standards as instructed.
2.Add 100 µl positive control, negative control, or sample to each well. Incubate 1.5 hours at room temperature.
3.Add 100 µl prepared HRP-anti positive control IgA Antibody and/or 100 µl of prepared HRP-anti Human IgA Antibody to each well. Incubate 1 hour at room temperature.
4.Add 100 ul TMB One-Step Substrate Reagent to each well. Incubate 10 minutes at room temperature.
5.Add 50 ul Stop Solution to each well. Read at 450 nm immediately.