|Product Name:||DnaK SBD Recombinant Protein|
|Synonyms:||HSP-70, HSP70, DnaK, Chaperone protein dnaK, Heat shock protein 70, Heat shock 70 kDa protein, groP, grpF, seg, b0014, JW0013.|
|Physical Appearance:||Sterile filtered colorless solution.|
|Formulation:||The protein contains 25mM Tris-HCl, pH7.5, 2mM B-ME and 1mM DTT.|
|Stability:||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.|
|Purity:||Greater than 95.0% as determined by SDS-PAGE.|
|Amino Acid Sequence:||MDVK DVLL LDVT PLSL GIET MGGV MTTL IAKN TTIP TKHS QVFS TAED NQSA VTIH VLQG ERKR AADN KSLG QFNL DGIN PAPR GMPQ IEVT FDID ADGI LHVS AKDK NSGK EQKI TIKA SSGL NEDE IQKM VRDA EANA EADR KFEE LVQT RNQG DHLL HST|
DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak(residues 508-638) of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft. DnaK(amino acid 508-638) was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus.
Recombinant DnaK Substrate Binding domain produced in E.Coli is a single, non-glycosylated polypeptide chain containing (385-546 a.a.) 163 amino acids and having a molecular mass of 17.7 kDa.