ESPL1 Rabbit Polyclonal Antibody (CAB15366)

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Technical Manual

ESPL1 Rabbit Polyclonal Antibody

Product Name:ESPL1 Rabbit Polyclonal Antibody
Product SKU:CAB15366
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1220-1500 of human ESPL1 (NP_036423.4).


Gene ID:9700
Swiss Prot:Q14674
Synonyms:ESPL1; ESP1; SEPA; separin
Calculated MW:197kDa/233kDa
Observed MW:233kDa
Reactivity:Human, Mouse, Rat
Application:WB IF
Recommended Dilution:WB 1:200 - 1:2000 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunofluorescence 
Positive Samples:HeLa, Jurkat, Mouse spleen, Rat spleen, Rat testis
Cellular Location:Cytoplasm, Nucleus

Product Images

ESPL1 Rabbit Polyclonal Antibody (CAB15366)

Western blot analysis of extracts of various cell lines, using ESPL1 antibody (CAB15366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.

Uniprot Information

UniProt Protein Function:separase: a caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms. Regulated by at least two independent mechanisms. First, it is inactivated via its interaction with securin/PTTG1, which probably covers its active site. Second, phosphorylation inactivates it. It is completely phosphorylated during mitosis and dephosphorylated during anaphase. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:Protease; EC

Chromosomal Location of Human Ortholog: 12q

Cellular Component: centrosome; nucleus; cytosol

Molecular Function:protein binding; catalytic activity; cysteine-type peptidase activity

Biological Process: establishment of mitotic spindle localization; homologous chromosome segregation; mitotic sister chromatid segregation; apoptosis; negative regulation of sister chromatid cohesion; positive regulation of mitotic metaphase/anaphase transition; cytokinesis; mitotic cell cycle; proteolysis; meiotic spindle organization and biogenesis

NCBI Summary:Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM, Nov 2010]
UniProt Code:Q14674
NCBI GenInfo Identifier:308153600
NCBI Gene ID:9700
NCBI Accession:Q14674.3
UniProt Related Accession:Q14674
Molecular Weight:2120
NCBI Full Name:Separin
NCBI Synonym Full Names:extra spindle pole bodies homolog 1 (S. cerevisiae)
NCBI Official Symbol:ESPL1 
NCBI Official Synonym Symbols:ESP1; SEPA 
NCBI Protein Information:separin; caspase-like protein ESPL1; extra spindle poles like 1; separin, cysteine protease; extra spindle poles-like 1 protein
UniProt Protein Name:Separin
UniProt Synonym Protein Names:Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase
Protein Family:Separin
UniProt Gene Name:ESPL1 
UniProt Entry Name:ESPL1_HUMAN
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Additional Information

Product type:
Host Species:
Antibody Type:
Polyclonal Antibody
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