GeniePlex Mouse Inflammation 7-Plex Panel 2 (96 Tests)



ELISA Kit Technical Manual

Product Name: Genieplex Mouse Inflammation 7-Plex Immunoassay Panel 2


IL-1-Alpha, IL-1-Beta, IL-6, IL-10, IL-22, IL-23, TNF-Alpha

Species Reactivity:


Assay Type:


Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex Genieplex Mouse Inflammation 7-Plex Immunoassay Panel 2 is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IL-1-Alpha, IL-1-Beta, IL-6, IL-10, IL-22, IL-23, TNF-Alpha on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 7 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Analytes:IL-1-Alpha, IL-1-Beta, IL-6, IL-10, IL-22, IL-23, TNF-Alpha
1x Ab-conjugated beads (premixed):S4P2 - anti-M IL-1-Beta; S4P3 - anti-M IL-23p19; S4P4 - anti-M TNF-Alpha; S4P6 - anti-M IL-1-Alpha; S5P5 - anti-M IL-6; S5P6 - anti-M IL-22; S5P9 - anti-M IL-10
2x Biotin-detection Ab (premixed):Biotin - anti-M IL-1-Beta; Biotin - anti-M IL-23p19; Biotin - anti-M TNF-Alpha; Biotin - anti-M IL-1-Alpha; Biotin - anti-M IL-6; Biotin - anti-M IL-22; Biotin - anti-M IL-10
Buffers:2x Mouse/Rat dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.
Lyophilized Standard Mix:Lyophilized Standard Mix-Mouse Inflammation 7-Plex Panel 2. One vial containing lyophilized recombinant IL-1-Alpha, IL-1 Beta, IL-6, IL-10, IL-22, IL-23, TNF-Alpha.
Sample types:Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs):IL-1-Alpha, < 2 pg/mL; IL-1-Beta, < 2 pg/mL;  IL-6, < 3 pg/mL; IL-10, < 2 pg/mL; IL-22, < 5 pg/mL; IL-23p19, < 5 pg/mL; TNF-Alpha, < 0.5 pg/mL.
LLOQs:IL-1-Alpha, < 10 pg/mL; IL-1-Beta, < 5 pg/mL;  IL-6, < 10 pg/mL; IL-10, < 5 pg/mL; IL-22, < 10 pg/mL; IL-23p19, < 10 pg/mL; TNF-Alpha, < 2 pg/mL.
ULOQs:IL-1-Alpha, > 5,000; IL-1-Beta, > 5,000 pg/mL;  IL-6, > 5,000 pg/mL; IL-10, > 2,000 pg/mL; IL-22, > 5,000 pg/mL; IL-23p19, > 5,000 pg/mL; TNF-Alpha, > 1,500 pg/mL..
Standard dose recovery:Standard dose recovery: 70-130%
Intra-assay CV:Intra-assay CV: < 10%
Inter-assay CV:Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume:Sample volume: 15 µL/test


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