Glucose Dehydrogenase Assay Kit (Colorimetric) (BA0024)
Glucose Dehydrogenase Assay Kit - InformationAssay Genie's non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
ApplicationsFor quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.
Glucose Dehydrogenase Assay Kit - Key Features
- Fast and sensitive. Linear detection range (20 sample): 0.5 to 200 U/L for 15 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Glucose Dehydrogenase Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL Diaphorase: 120 uL NAD/MTT: 1 mL Calibrator: 1.5 mL Substrate: 1 mL|
|Kit Requires||Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat- bottom 96-well plates, centrifuge tubes and plate reader.|
|Method of Detection||OD565nm|
|Detection Limit||0.5 U/L|
|Samples||Biological samples (e.g. plasma, serum, tissue and culture media).|
|Protocol Length||15 min|
|Shelf Life||6 months|
|Storage||Store all components at -20°C upon receiving|
More DetailsGLUCOSE DEHYDROGENASE (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.