GPX4 Antibody (PACO50106)
- SKU:
- PACO50106
- Product type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Application:
- ELISA
- Application:
- WB
- Application:
- IHC
- Application:
- IF
- Antibody type:
- Polyclonal
- Conjugation:
- Unconjugated
Description
| Antibody Name: | GPX4 Antibody (PACO50106) |
| Antibody SKU: | PACO50106 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant Human Phospholipid hydroperoxide glutathione peroxidase, mitochondrial protein (74-197AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, Mouse kidney tissue, Rat heart tissue. All lanes: GPX4 antibody at 3.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 23, 20 kDa. Observed band size: 23 kDa.. |
 | Immunofluorescent analysis of A549 cells using PACO50106 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | Immunohistochemistry of paraffin-embedded human kidney tissue using PACO50106 at dilution of 1:100. |
| Background: | Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T cell response to viral and parasitic infection by protecting T cells from ferroptosis, a cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species, and by supporting T cell expansion. |
| Synonyms: | Phospholipid hydroperoxide glutathione peroxidase (PHGPx) (EC 1.11.1.12) (Glutathione peroxidase 4) (GPx-4) (GSHPx-4), GPX4 |
| UniProt Protein Function: | GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation. |
| UniProt Protein Details: | Protein type:Other Amino Acids Metabolism - glutathione; Nuclear envelope; Lipid Metabolism - arachidonic acid; EC 1.11.1.12; Oxidoreductase; Mitochondrial Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: mitochondrion; nucleus; cytosol Molecular Function:selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity Biological Process: glutathione metabolic process; lipoxygenase pathway; hydrogen peroxide catabolic process; multicellular organismal development; phospholipid metabolic process; regulation of inflammatory response; arachidonic acid metabolic process; response to oxidative stress; response to estradiol stimulus; aging |
| NCBI Summary: | This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. The encoded protein has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | P36969 |
| NCBI GenInfo Identifier: | 172045844 |
| NCBI Gene ID: | 2879 |
| NCBI Accession: | P36969.3 |
| UniProt Secondary Accession: | P36969,O43381, Q6PJ59, Q9UPK2, |
| UniProt Related Accession: | P36969 |
| Molecular Weight: | Observed MW: 22kDaCalculated MW: 19kDa/22kDa |
| NCBI Full Name: | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial |
| NCBI Synonym Full Names: | glutathione peroxidase 4 |
| NCBI Official Symbol: | GPX4 |
| NCBI Official Synonym Symbols: | MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx |
| NCBI Protein Information: | phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase |
| UniProt Protein Name: | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial |
| UniProt Synonym Protein Names: | Glutathione peroxidase 4; GPx-4; GSHPx-4 |
| Protein Family: | Probable glutathione peroxidase |
| UniProt Gene Name: | GPX4 |
| UniProt Entry Name: | GPX4_HUMAN |
| Antibodies |
| Anti-GPX4 Antibody (CAB13309) |
| Anti-GPX4 Antibody (CAB11243) |
| Anti-GPX4 Antibody (CAB1933) |
| GPX4 Antibody (PACO09577) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
