Human Heat Shock Protein 27, His Tag Recombinant Protein (CPRT5236)

SKU:
CPRT5236
€278

Description

Data SheetMSDS

HSP27 Human, His Recombinant Protein

Name:HSP27 Human, His
Synonyms:HSPB1, CMT2F, HMN2B, HSP27, HSP28, HSP25, Heat shock protein beta-1, Heat shock 27 kDa protein, Stress-responsive protein 27, SRP27, HS.76067, DKFZp586P1322.
Introduction:HSP27 (also known as the Estrogen–Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress includingelevated temperatures, toxic metals, drugs and oxidants. Also, Hsp27 is phosphorylated in vivo on three phosphorylation sites (Ser15, Ser78 and Ser82) by protein kinases includingMAPKAP kinase 2 and the stress–activated protein kinase SAPK2 (p38).
Description:Recombinant Human HSP–27 produced in E.Coli is a single, non–glycosylated polypeptide chain containing 225 amino acids (1–205 a.a.) and having a molecular mass of 24.9kDa. Hsp27 is fused to a 20 amino acid his tag at N–terminus and purified by conventional chromatography.
Source:Escherichia Coli.
Pack size:25µg
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The HSP27 protein solution contains 20mM Tris–Hcl buffer, 1mM DTT and 10% glycerol.
Solubility:N/A
Stability:Store at 4°C if entire vial will be used within 2–4 weeks. Store, frozen at –20°C for longer periods of time. For longterm storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze–thaw cycles.
Purity:Greater than 95.0% as determined by SDS–PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK.
Biological Activity:N/A
Usage:Assay Genie’s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Uniprot:P04792
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Additional Information

Product type:
Recombinant Protein
Reactivity:
Human
Host Species:
E.Coli
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