Description
| Product Name: | Human VAV1 Recombinant Protein |
| Product Code: | RPPB5114 |
| Size: | 10µg |
| Species: | Human |
| Target: | VAV1 |
| Synonyms: | Vav 1 Guanine Nucleotide Exchange Factor, VAV, Vav 1Oncogene, Proto-Oncogene Vav. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | VAV1 protein solution (0.25mg/ml) containing In 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 30%glycerol and 2mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles. |
| Purity: | Greaterthan 85% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMTEYDKR CCCLREIQQT EEKYTDTLGS IQQHFLKPLQ RFLKPQDIEIIFINIEDLLR VHTHFLKEMK EALGTPGAAN LYQVFIKYKE RFLVYGRYCS QVESASKHLD RVAAAREDVQMKLEECSQRA NNGRFTLRDL LMVPMQRVLK YHLLLQELVK HTQEAMEKEN LRLALDAMRD LAQCVNEVKR DNETLRQITN FQLSIENLDQSLAHYGRPKI DGELKITSVE RRSKMDRYAF LLDKALLICK RRGDSYDLKD FVNLHSFQVR DDSSGDRDNKKWSHMFLLIE DQGAQGYELF FKTRELKKKW MEQFEMAISN IYPENATANG HDFQMFSFEE TTSCKACQML LRGTFYQGYR CHRCRASAHK ECLGRVPPCG |
Vav1 Guanine Nucleotide Exchange Factor (VAV1), belongs to the VAV gene family.The VAV1 proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases whichactivate pathways leading to actin cytoskeletal rearrangements and transcriptionalalterations. VAV1 is essential in hematopoiesis, taking part in T-cell andB-cell development and activation. VAV1 has been identified as the specificbinding partner of Nef proteins from HIV-1. In addition, VAV1 contains an SH2domain, which could show its function as a substrate for tyrosine kinases. Expressionof VAV1 is limited solely to cells of hematopoietic origin, as well as those ofthe erythroid, lymphoid and myeloid lineages. Among the diseases associatedwith VAV1 are cherubism, and hiv-1.
VAV1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 400 amino acids (189-565a.a) and having a molecular mass of 46.8kDa.VAV1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | VAV1: a proto-oncogenic guanine nucleotide exchange factors (GEF) of the Dbl family. A GEF for the Rho family of GTP binding proteins. Expressed exclusively in hematopoietic cells. Important in hematopoiesis, playing a role in T-cell and B-cell development and activation. |
| UniProt Protein Details: | Protein type:GEFs, Rac/Rho; GEFs; Oncoprotein; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: plasma membrane; intercellular junction; cytosol Molecular Function:protein binding; metal ion binding; Rac guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; transcription factor activity Biological Process: epidermal growth factor receptor signaling pathway; integrin-mediated signaling pathway; neutrophil chemotaxis; platelet activation; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; T cell activation; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; G-protein coupled receptor protein signaling pathway; regulation of small GTPase mediated signal transduction; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction; T cell costimulation; innate immune response; blood coagulation; vascular endothelial growth factor receptor signaling pathway |
| NCBI Summary: | This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012] |
| UniProt Code: | P15498 |
| NCBI GenInfo Identifier: | 13124807 |
| NCBI Gene ID: | 7409 |
| NCBI Accession: | P15498.4 |
| UniProt Secondary Accession: | P15498,Q15860, B4DVK9, M0QXX6, |
| UniProt Related Accession: | P15498 |
| Molecular Weight: | 845 |
| NCBI Full Name: | Proto-oncogene vav |
| NCBI Synonym Full Names: | vav 1 guanine nucleotide exchange factor |
| NCBI Official Symbol: | VAV1�� |
| NCBI Official Synonym Symbols: | VAV�� |
| NCBI Protein Information: | proto-oncogene vav; vav 1 oncogene |
| UniProt Protein Name: | Proto-oncogene vav |
| Protein Family: | Proto-oncogene |
| UniProt Gene Name: | VAV1�� |
| UniProt Entry Name: | VAV_HUMAN |
