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Human, Mouse and Rat Phosphotyrosine MEK1 PharmaGenie ELISA Kit (SBRS1890)

SKU:
SBRS1890
Product type:
ELISA Kit
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Application:
ELISA
ELISA Type:
Sandwich
  • Human, Mouse and Rat Phosphotyrosine MEK1 PharmaGenie ELISA Kit SBRS1890
  • Human, Mouse and Rat Phosphotyrosine MEK1 PharmaGenie ELISA Kit SBRS1890
€635
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Description

Human, Mouse and Rat Phosphotyrosine MEK1 PharmaGenie ELISA - Kit Data

Product SKU:SBRS1890
Size:96T
Application:The antibody pair provided in this kit recognizes human tyrosine-phosphorylated MEK1.
Uniprot:P31938 / Q01986 / Q02750
Gene ID:5604
Pathway:Tyrosine Kinase Family
Synonyms:Dual specificity mitogen-activated protein kinase kinase 1 (MAP kinase kinase 1) (MAPKK 1) (MKK1) (EC 2.7.12.2) (ERK activator kinase 1) (MAPK/ERK kinase 1) (MEK 1)
Target Species:Human, Mouse, Rat
Compatible Sample Types:Cell Lysates, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Semi-Quantitative
Storage/Stability:Upon receipt, the kit should be stored at -20°C. Please use within 6 months from the date of shipment.

Human, Mouse and Rat Phosphotyrosine MEK1 PharmaGenie ELISA Kit (SBES1890) - Product Images

Product ImageHeLa cells were untreated or treated with Pervanadate for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA:
Product ImageHeLa cells were treated with Pervanadate at 37°C for 10 min. Cells were solubilzed at 4 x 107 cells/ml in lysis buffer. Serial dilutions of lysates were analyzed in this ELISA

Kit Components:

1.Pre-Coated 96-well Strip Microplate
2.Wash Buffer
3.Biotinylated Anti-Phosphotyrosine Antibody
4.Stop Solution
5.Assay Diluent(s)
6.Positive Control Sample
7.Lysis Buffer
8.Streptavidin-Conjugated HRP
9.TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human, Mouse and Rat Phosphotyrosine MEK1 ELISA Kit will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 ul to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm

Kit Protocol:

1.Prepare all reagents and samples as instructed in the manual.
2.Add 100 ul of sample or positive control to each well.
3.Incubate 2.5 h at RT or O/N at 4 °C.
4.Add 100 ul of prepared primary antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 ul of prepared 1X HRP-Streptavidin to each well.
7.Incubate 1 h at RT.
8.Add 100 ul of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 ul of Stop Solution to each well.
11.Read at 450 nm immediately.