Human MTHFD2 Recombinant Protein (RPPB1983)
- SKU:
- RPPB1983
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P13995
- Research Area:
- Enzymes
Description
| Product Name: | Human MTHFD2 Recombinant Protein |
| Product Code: | RPPB1983 |
| Size: | 20µg |
| Species: | Human |
| Target: | MTHFD2 |
| Synonyms: | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial, NAD-dependent methylenetetrahydrofolate dehydrogenase, Methenyltetrahydrofolate cyclohydrolase, NMDMC, MTHFD2. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The MTHFD2 solution (1mg/ml) contains Phosphate buffered saline (pH7.4), 20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSLAAVRNE AVVISGRKLA QQIKQEVRQE VEEWVASGNK RPHLSVILVG ENPASHSYVL NKTRAAAVVG INSETIMKPA SISEEELLNL INKLNNDDNV DGLLVQLPLP EHIDERRICN AVSPDKDVDG FHVINVGRMC LDQYSMLPAT PWGVWEIIKR TGIPTLGKNV VVAGRSKNVG MPIAMLLHTD GAHERPGGDA TVTISHRYTP KEQLKKHTIL ADIVISAAGI PNLITADMIK EGAAVIDVGI NRVHDPVTAK PKLVGDVDFE GVRQKAGYIT PVPGGVGPMT VAMLMKNTII AAKKVLRLEE REVLKSKELG VATN |
MTHFD2plays a role as a homodimerwhich requires magnesium and inorganic phosphate. MTHFD2 has a pseudogene onchromosome 7 and owns 3 different enzymatic activities. Each of the activitiescatalyzes 1 of 3 sequential reactions in the interconversion of 1-carbonderivatives of tetrahydrofolate, which are substrates for methionine,thymidylate, and de novo purine syntheses.
MTHFD2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 344 amino acids (30-350) and having a molecular mass of 37.2kDa.MTHFD2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | MTHFD2: a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009] |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.5.4.9; Hydrolase; Oxidoreductase; EC 1.5.1.15; Mitochondrial; Cofactor and Vitamin Metabolism - one carbon pool by folate Chromosomal Location of Human Ortholog: 2p13.1 Cellular Component: cytosol; extracellular space; mitochondrial matrix; mitochondrion Molecular Function:formate-tetrahydrofolate ligase activity; magnesium ion binding; methenyltetrahydrofolate cyclohydrolase activity; methylenetetrahydrofolate dehydrogenase (NAD+) activity; methylenetetrahydrofolate dehydrogenase (NADP+) activity; phosphate binding Biological Process: folic acid metabolic process; one-carbon compound metabolic process; tetrahydrofolate metabolic process |
| NCBI Summary: | This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq, Mar 2009] |
| UniProt Code: | P13995 |
| NCBI GenInfo Identifier: | 115311607 |
| NCBI Gene ID: | 10797 |
| NCBI Accession: | P13995.2 |
| UniProt Secondary Accession: | P13995,Q53G90, Q53GV5, Q53S36, Q7Z650, |
| UniProt Related Accession: | P13995 |
| Molecular Weight: | 26,849 Da |
| NCBI Full Name: | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial |
| NCBI Synonym Full Names: | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase |
| NCBI Official Symbol: | MTHFD2�� |
| NCBI Official Synonym Symbols: | NMDMC�� |
| NCBI Protein Information: | bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial |
| UniProt Protein Name: | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial |
| Protein Family: | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase |
| UniProt Gene Name: | MTHFD2�� |
| UniProt Entry Name: | MTDC_HUMAN |
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