The Human TE (Telomerase) ELISA Kit is specially designed for the precise measurement of telomerase levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an excellent choice for various research purposes.Telomerase is a key enzyme that plays a vital role in maintaining the length of telomeres, which are protective caps at the end of chromosomes. Telomerase activity is closely linked to cellular aging, cancer development, and other age-related diseases. Therefore, detecting telomerase levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Human TE (Telomerase) ELISA Kit is a valuable tool for researchers studying telomerase biology, aging, cancer, and other related fields. Its reliability and precision make it an essential instrument for advancing scientific understanding and discovery.
Product Name:
Human TE (Telomerase) ELISA Kit
SKU:
HUES01434
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
96T/48T/24T: 2 vials, 20 mL | 96T*5: 10 vials, 20 mL
2–8°C, 12 months
Biotinylated Detection Ab Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
HRP Conjugate Diluent
96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL
2–8°C, 12 months
Concentrated Wash Buffer(25×)
96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL
2–8°C, 12 months
Substrate Reagent
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C (Protect from light)
Stop Solution
96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL
2–8°C
Plate Sealer
96T/48T/24T: 5 pieces | 96T*5: 25 pieces
2–8°C
Technical Manual
1 copy
-
Certificate of Analysis
1 copy
-
Linearity:
Serum (n=5)
EDTA Plasma (n=5)
Cell Culture Media (n=5)
1:2
Range (%)
94-108
96-110
90-104
Average (%)
101
103
98
1:4
Range (%)
92-107
85-100
84-99
Average (%)
98
92
91
1:8
Range (%)
90-102
88-101
81-93
Average (%)
97
93
87
1:16
Range (%)
89-104
84-94
84-95
Average (%)
96
89
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
EDTA Plasma (n=8)
89-105
96
Cell Culture Media (n=8)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.07
3.79
0.44
1.03
3.92
Standard deviation
0.02
0.07
0.22
0.03
0.07
0.26
C V (%)
5.12
6.84
5.93
6.34
6.97
6.61
Uniport ID:
O14746
Sample type & Sample volume:
Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:
This kit recognizes Human TE in samples. No significant cross-reactivity or interference between Human TE and analogues was observed
Storage:
2-8℃
Research Area:
Cancer,Stem Cells,Immunology,Metabolism,Cell Biology,Cardiovascular,Signal Transduction,Neuroscience,Tags And Cell Markers,Epigenetics And Nuclear Signal
