Anti-KDM3A Antibody (CAB2322)

Antibody Name:	Anti-KDM3A Antibody
Antibody SKU:	CAB2322
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KDM3A (NP_060903.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human KDM3A (NP_060903.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVLT LGES WPVL VGRR FLSL SAAD GSDG SHDS WDVE RVAE WPWL SGTI RAVS HTDV TKKD LKVC VEFD GESW RKRR WIEV YSLL RRAF LVEH NLVL AERK SPEI SERI VQWP AITY KPLL DKAG LGSI TSVR FLGD QQRV FLSK DLLK PIQD VNSL RLSL TDNQ IVSK EFQA LIVK HLDE SHLL KGDK NLVG SEVK IYSL DPST QWFS ATVI NGNP ASKT LQVN CEEI PALK IVDP SLIH VEVV HDNL VTCG NSAR IGAV KRKS SENN GTLV SKQA KSCS EASP SMCP VQSV PTTV FKEI
Gene ID:	55818
Uniprot:	Q9Y4C1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	147kDa
Observed MW:	157kDa

Synonyms:	KDM3A, JHDM2A, JHMD2A, JMJD1, JMJD1A, TSGA
Background:	This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	JMJD1A: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. Belongs to the JHDM2 histone demethylase family.
UniProt Protein Details:	Protein type:EC 1.14.11.-; Demethylase; Oxidoreductase; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus Molecular Function:androgen receptor binding; dioxygenase activity; iron ion binding; transcription factor activity Biological Process: negative regulation of histone H3-K9 methylation; formaldehyde biosynthetic process; establishment and/or maintenance of chromatin architecture; spermatid nuclear elongation; histone H3-K9 demethylation; hormone-mediated signaling; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniProt Code:	Q9Y4C1
NCBI GenInfo Identifier:	308153659
NCBI Gene ID:	55818
NCBI Accession:	Q9Y4C1.4
UniProt Related Accession:	Q9Y4C1
Molecular Weight:	147kDa
NCBI Full Name:	Lysine-specific demethylase 3A
NCBI Synonym Full Names:	lysine demethylase 3A
NCBI Official Symbol:	KDM3A
NCBI Official Synonym Symbols:	TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
NCBI Protein Information:	lysine-specific demethylase 3A
UniProt Protein Name:	Lysine-specific demethylase 3A
UniProt Synonym Protein Names:	JmjC domain-containing histone demethylation protein 2A; Jumonji domain-containing protein 1A
Protein Family:	Lysine-specific demethylase
UniProt Gene Name:	KDM3A
UniProt Entry Name:	KDM3A_HUMAN

Western blot analysis of extracts of BT-474 cells, using KDM3A antibody (CAB2322) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

Antibodies	ELISA Kits
Anti-KDM3A Antibody (CAB11960)	Human KDM3A/JMJD1A ELISA Kit (HUFI03731)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)