Description
Lipoxygenase (LOX) Activity Fluorometric Assay Kit (MAES0320)
The Lipoxygenase (LOX) Activity Fluorometric Assay Kit is a sensitive and reliable tool designed for the quantitative detection of LOX enzyme activity in various biological samples, including serum, plasma, animal tissues, and cultured cells. LOX is an iron-containing oxidoreductase involved in the oxidation of polyunsaturated fatty acids, playing a critical role in inflammation, oxidative stress, and ferroptosis pathways. This kit employs a fluorometric detection method that measures the conversion of arachidonic acid to fluorescent products, providing accurate and reproducible results with a detection sensitivity as low as 0.05 U/L.
The assay is optimized for use with a wide range of sample types, including human and rat serum, mouse tissue homogenates, and HL-60 or Jurkat cells, with flexible dilution references to ensure compatibility. The assay requires only 10 µL of sample and can be completed in approximately 50 minutes, making it suitable for high-throughput screening. With a broad detection range (0.05–0.50 U/L) and excellent assay precision (intra-assay CV: 2.2–4.9%, inter-assay CV: 6.4–9.4%), this kit is ideal for researchers investigating LOX-related biological processes and diseases such as cancer, neurodegeneration, and inflammatory disorders.
| Product Name: | Lipoxygenase (LOX) Activity Fluorometric Assay Kit |
| SKU: | MAES0320 |
| Size: | 96 Assays |
| Detection Method: | Fluorometric Method |
| Senitivity: | 0.05 U/L |
| Range: | 0.05-0.50 U/L |
| Sample Type: | Serum, Plasma, Animal tissue, Cell |
| Detection Instrument: | Fluorescence microplate reader (Ex/Em=507 nm/547 nm) |
| Research Area: | Ferroptosis |
| Other Reagents Required: | Normal saline (0.9% NaCl) |
| Storage: | This product can be stored at -20°C for 12 months with shading light. |
| Precision: | Inter-assay CV: 6.4-9.4% Intra-assay CV: 2.2-4.9% |
| Sample Volume: | 10 μL |
| Assay Time: | 50 minutes |
| Sample Dilution Reference: |
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Lipoxygenase (LOX) is an iron-containing oxidoreductase present in both plants and animals. This assay kit detects LOX activity based on its ability to catalyze the oxidation of arachidonic acid, resulting in the formation of fluorescent products. LOX enzyme activity is quantified by measuring the change in fluorescence intensity of these products over time, using an excitation wavelength of 507 nm and an emission wavelength of 547 nm.
