null

Rat TNF-alpha ELISpot Pair

SKU:
RTDC0184
Product type:
ELISpot
Reactivity:
Rat
€670
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS

Rat TNF-alpha ELISpot Pair

Assay Genie ELISpot is a highly specific immunoassay for the analysis of TNF-α production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of TNF-α producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. Utilising sandwich immuno-enzyme technology, Assay Genie ELISpot assays can detect both secreted TNF-α (qualitative analysis) and single cells that produce TNF-α (quantitative analysis). Cell secreted TNF-α is captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured TNF-α is revealed by tracer antibodies and appropriate conjugates.