Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.
Product Name:
Human Biotinidase/BTD Recombinant Protein (RPES3578)
Product Code:
RPES3578
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
BTD
Accession:
P43251
Sequence:
Met 1-Asp543
Fusion tag:
C-His
Activity:
Measured by its ability to hydrolyze biocytin to lysine and biotin. The specific activity is >500pmol/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human BTD (P43251) (Met1-Asp543) with a C-terminal polyhistidine tag was expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
58.2 kDa
AP Mol Mass:
66-76 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
