The cluster of differentiation (CD) system is a popular tool for immunophenotyping. The surface CD molecules that interact with the cell's immune function can be used to distinguish various types of cells in the immune system. There are more than 320 unique CD clusters and subsets have been discovered. Certain CD molecules act as receptors or ligands that are crucial to the cell, triggering a signal cascade that changes the cell's behavior. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Neprilysin (CD10), also known as neutral endopeptidase, is a member of the CD system. CD10 is a zinc-dependent metalloprotease enzyme that has been found to break down a variety of small secreted peptides, including the amyloid beta peptide. It's present in the kidney and lung tissues at a high level as a membrane-bound protein. The CD10 protein has been linked to Alzheimer's disease, with mutations in the gene causing familial Alzheimer's disease. Other chemical processes are also affected by CD10. Human CD10/Neprilysin Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human CD10/Neprilysin Recombinant Protein (RPES2866)
Product Code:
RPES2866
Size:
10µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
CALLA,CD10,NEP,SFE
Accession:
NP_000893.2
Sequence:
Tyr 52-Trp 750
Fusion tag:
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH, (R&D Systems, Catalog # ES005). The specific activity is >1,000 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Tyr 52-Trp 750) of human MME (NP_000893.2) was expressed and purified.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
80 kDa
AP Mol Mass:
90-100 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
