Cytotoxic Tlymphocyte 4(CTLA-4;CD152); is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence; a 126 aa extracellular domain (ECD) with one Ig-like V-type domain; a 21 aa transmembrane (TM) sequence; and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.
Human CTLA4 Recombinant Protein (RPES3191)
Cytotoxic T-lymphocyte protein 4,Cytotoxic T-lymphocyte-associated antigen 4,CTLA4,CD152,Cytotoxic T-Lymphocyte-Associated Protein 4
Immobilized Mouse B7-1-Fc(Cat: PKSM041366) at 10µg/ml(100 ?l/well) can bind Human CTLA-4-His. The ED50 of Human CTLA-4-His is 2.3 ng/ml .
<1.0 EU per µg as determined by the LAL method.
Recombinant Human Cytotoxic CTLA4 is produced by our Mammalian expression system and the target gene encoding Lys36-Asp161 is expressed with a 6His tag at the C-terminus.
> 95% as determined by reducing SDS-PAGE.
AP Mol Mass:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.