Recombinant Human Eotaxin-3/CCL26 Protein (aa 27-94) (RPES4843)

SKU:
RPES4843
€167

Description

ELISA Kit Technical Manual

Recombinant Human Eotaxin-3/CCL26 Protein (aa 27-94)

Background:Chemokine (C C Motif) Ligand 26 (CCL26) is a novel small cytokine belonging to the CC chemokine family, which involved in immunoregulatory and inflammatory processes. CCL26 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. It specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. Eotaxin-3/CCL26, along with Eotaxin and Eotaxin-2, selectively activates the CC chemokine receptor 3 (CCR3). The Eotaxin-3-CCR3 interaction may play an important role in allergic diseases such as atopic dermatitis and bronchial asthma. The full-length cDNA for Eotaxin-3 encodes a protein of 94 amino acids with a putative signal peptide of either 23 or 26 amino acid residues. Both the 71 and 68 amino acid residue variants of recombinant Eotaxin-3 demonstrate equal potency in inducing chemotaxis of a human CCR3-transfected cell line. Unlike most other CC chemokines, Eotaxin-3 maps to human chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. Eotaxin-3 and Eotaxin-2 are unique in that they are the only chemokines identified to date that map to chromosome 7.
Name:Recombinant Human Eotaxin-3/CCL26 Protein (aa 27-94)
Size:10µg
Assay Genie SKU:RPES4843
Synonyms:C-C Motif Chemokine 26; CC Chemokine IMAC; Eotaxin-3; Macrophage Inflammatory Protein 4-Alpha; MIP-4-Alpha; Small-Inducible Cytokine A26; Thymic Stroma Chemokine; TSC; CCL26; SCYA26
Species:Human
Expression host:E.coli
Sequence:Ser27-Leu94
Accession:Q9Y258
Mol Mass:8.2 kDa
AP Mol Mass:13 kDa
Tag:
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:CCL26: Chemotactic for eosinophils and basophils. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.
UniProt Protein Details:

Protein type:Chemokine; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: extracellular space

Molecular Function:chemokine activity

Biological Process: cell-cell signaling; positive regulation of actin filament polymerization; positive regulation of endothelial cell proliferation; immune response; chemotaxis; signal transduction; inflammatory response; positive regulation of cell migration

NCBI Summary:This gene is one of two Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 7. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for normal peripheral blood eosinophils and basophils. The product of this gene is one of three related chemokines that specifically activate chemokine receptor CCR3. This chemokine may contribute to the eosinophil accumulation in atopic diseases. [provided by RefSeq, Jul 2008]
UniProt Code:Q9Y258
NCBI GenInfo Identifier:6685990
NCBI Gene ID:10344
NCBI Accession:Q9Y258.1
UniProt Secondary Accession:Q9Y258,Q52LV8, A0N0Q5,
UniProt Related Accession:Q9Y258
Molecular Weight:
NCBI Full Name:C-C motif chemokine 26
NCBI Synonym Full Names:chemokine (C-C motif) ligand 26
NCBI Official Symbol:CCL26
NCBI Official Synonym Symbols:IMAC; TSC; MIP-4a; SCYA26; MIP-4alpha
NCBI Protein Information:C-C motif chemokine 26; eotaxin-3; MIP-4-alpha; chemokine N1; CC chemokine IMAC; thymic stroma chemokine; small inducible cytokine A26; small-inducible cytokine A26; macrophage inflammatory protein 4-alpha; small inducible cytokine subfamily A (Cys-Cys), member 26
UniProt Protein Name:C-C motif chemokine 26
UniProt Synonym Protein Names:CC chemokine IMAC; Eotaxin-3; Macrophage inflammatory protein 4-alpha; MIP-4-alpha; Small-inducible cytokine A26; Thymic stroma chemokine; TSC
Protein Family:C-C motif chemokine
UniProt Gene Name:CCL26
UniProt Entry Name:CCL26_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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