Human Erythropoietin R/EPOR Recombinant Protein (RPES2856)

SKU:
RPES2856
Product type:
Recombinant Protein
Host species:
Human Cells
Reactivity:
Human
€129

Description

ELISA Kit Technical Manual

Recombinant Human Erythropoietin R/EPOR Protein (His Tag)

Background:Erythropoietin (Epo), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of Epo are mediated by the erythropoietin receptor (Epo R). The presence of a soluble form of the Epo R has also been detected on human sera. Recombinant soluble Epo R binds Epo with high affinity and is a potent Epo antagonist.
Name:Recombinant Human Erythropoietin R/EPOR Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES2856
Synonyms:EpoR; EPO-R; Erythropoietin R; erythropoietin receptor; MGC138358; Platelet Receptor EPO-R
Species:Human
Expression host:Human Cells
Sequence:Ala25-Pro250
Accession:P19235
Mol Mass:28.4 kDa
AP Mol Mass:28-38 kDa
Tag:C-His
Purity:> 95% as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per ?g as determined by the LAL method.
Storage:Lyophilized protein should be stored at < -20'C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7? for 2-7 days.Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS,pH 7.4.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:EPOR: erythropoietin receptor: a member of the cytokine receptor family. Mediates erythropoietin-induced erythroblast proliferation, differentiation and survival. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. Forms homodimers on EPO stimulation. Tyrosine-phosphorylated EpoR may bind several SH2 domain-containing proteins including LYN, the adapter protein APS, SHP, SHP-2, JAK2, PI3 kinases, STAT5A/B, SOCS3, and CRKL. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Three alternatively-spliced isoforms have been described. Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.
UniProt Protein Details:

Protein type:Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: integral to plasma membrane

Molecular Function:erythropoietin receptor activity; identical protein binding; protein binding

Disease: Erythrocytosis, Familial, 1

NCBI Summary:This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Code:P19235
NCBI GenInfo Identifier:119524
NCBI Gene ID:2057
NCBI Accession:P19235.1
UniProt Secondary Accession:P19235,Q15443, Q2M205, B2RCG4,
UniProt Related Accession:P19235
Molecular Weight:35,809 Da
NCBI Full Name:Erythropoietin receptor
NCBI Synonym Full Names:erythropoietin receptor
NCBI Official Symbol:EPOR
NCBI Official Synonym Symbols:EPO-R
NCBI Protein Information:erythropoietin receptor
UniProt Protein Name:Erythropoietin receptor
Protein Family:Erythropoietin receptor
UniProt Gene Name:EPOR
UniProt Entry Name:EPOR_HUMAN