Interleukin-8 (IL-8), also known as CXCL8, is a chemokine with a characteristic CXC amino acid sequence that was discovered for its leukocyte chemotactic activity and is now recognized to have tumorigenic and proangiogenic activities. This cytokine is released by a range of cell types, including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory triggers (IL1, TNF, LPS , etc). IL-8 is produced and secreted at high levels in tumors of humans and mice, particularly gliomas. Recent studies suggest that it is important to glial tumor neovascularity and progression, both in vitro and in vivo. Levels of IL-8 correlate with histologic grade in glial neoplasms, and the most malignant form, glioblastoma, shows the highest expression in pseudopalisading cells around necrosis, suggesting that hypoxia/anoxia may stimulate expression. Interleukin (IL)-8/CXCL8 is a potent neutrophil chemotactic factor. Various stimuli, including proinflammatory cytokines, microbes and their by-products, and environmental changes such as hypoxia, reperfusion, and hyperoxia, have all been linked to IL-8/CXCL8 synthesis. IL-8/CXCL8 has been identified as a key mediator in neutrophil-mediated acute inflammation due to its strong effects on neutrophils. However, several lines of evidence suggest that IL-8/CXCL8 has a variety of effects on a number of cell types, including lymphocytes, monocytes, endothelial cells, and fibroblasts, in addition to neutrophils. The discovery of these biological actions implies that IL-8/CXCL8 has important functions in a variety of disease states, such as chronic inflammation and cancer. In breast cancer, IL-8 has been linked with tumor angiogenesis, metastasis, and poor survival. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.Human IL-8/CXCL8 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
A DNA sequence encoding the human IL8 (NP_000575.1) (Ser28-Ser99) was expressed with an initial Met.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
8.5 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
