Human Serum Amyloid A1/SAA1 Recombinant Protein (RPES4867)

SKU:
RPES4867
Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
€196

Description

ELISA Kit Technical Manual

Recombinant Human Serum Amyloid A1/SAA1 Protein (His Tag)

Background:Serum Amyloid A1 Protein (SAA1) is an acute phase apolipoprotein reactant that is produced predominantly by hepatocytes and is under the regulation of inflammatory cytokines. SAA is produced mainly in the liver and circulates in low levels in the blood. SAA may play a role in the immune system and facilitate the repair of injured tissues, it also acts as an antibacterial agent, and signals the migration of germ-fighting cells to sites of infection. SAA also functions as an apolipoprotein of the HDL complex. The SAA cleavage product designated amyloid protein A is deposited systemically as amyloid in vital organs such as the liver, spleen, and kidneys in chronic inflammatory diseases patients. These deposits are extremely insoluble and resistant to proteolysis; they disrupt tissue structure and compromise performance.
Name:Recombinant Human Serum Amyloid A1/SAA1 Protein (His Tag)
Size:10µg
Assay Genie SKU:RPES4867
Synonyms:Serum Amyloid A Protein; SAA; SAA1
Species:Human
Expression host:E.coli
Sequence:Arg19-Tyr122
Accession:P0DJI8
Mol Mass:13.2 kDa
AP Mol Mass:14 kDa
Tag:N-6His
Purity:> 95 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl,150mM NaCl,1mM EDTA, pH 8.0.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:SAA1: Major acute phase reactant. Apolipoprotein of the HDL complex. Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA1 protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function. Elevated serum SAA1 protein levels may be associated with lung cancer. Belongs to the SAA family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: extracellular region

Molecular Function:heparin binding; G-protein-coupled receptor binding

Biological Process: positive regulation of interleukin secretion; receptor-mediated endocytosis; platelet activation; neutrophil chemotaxis; elevation of cytosolic calcium ion concentration; positive regulation of cell adhesion; regulation of protein secretion; negative regulation of inflammatory response; acute-phase response; innate immune response; lymphocyte chemotaxis; macrophage chemotaxis; positive regulation of cytokine secretion

NCBI Summary:This gene encodes a member of the serum amyloid A family of apolipoproteins. The encoded protein is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimer's disease and Crohn's disease. This protein may also be a potential biomarker for certain tumors. Alternate splicing results in multiple transcript variants that encode the same protein. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Jun 2012]
UniProt Code:P0DJI8
NCBI GenInfo Identifier:395406826
NCBI Gene ID:6288
NCBI Accession:P0DJI8.1
UniProt Secondary Accession:P0DJI8,P02735, P02736, P02737, Q16730, Q16834, Q16835 Q16879, Q3KRB3, Q6FG67, Q96QN0, Q9UCK9,
UniProt Related Accession:P0DJI8
Molecular Weight:122
NCBI Full Name:Serum amyloid A protein
NCBI Synonym Full Names:serum amyloid A1
NCBI Official Symbol:SAA1
NCBI Official Synonym Symbols:SAA; PIG4; SAA2; TP53I4
NCBI Protein Information:serum amyloid A protein; serum amyloid A protein; tumor protein p53 inducible protein 4
UniProt Protein Name:Serum amyloid A protein
UniProt Synonym Protein Names:Amyloid fibril protein AA
UniProt Gene Name:SAA1
UniProt Entry Name:SAA1_HUMAN
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