Human TFIIB/GTF2B Recombinant Protein (RPES1173)

Product type:
Recombinant Protein
Host species:


ELISA Kit Technical Manual

Recombinant Human TFIIB/GTF2B Protein (GST Tag)

Background:Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.
Name:Recombinant Human TFIIB/GTF2B Protein (GST Tag)
Assay Genie SKU:RPES1173
Synonyms:Transcription Initiation Factor IIB; General Transcription Factor TFIIB; S300-II; GTF2B; TF2B; TFIIB
Expression host:E.coli
Sequence:Met 1-Leu316
Mol Mass:61.6 kDa
AP Mol Mass:62 kDa
Purity:> 85 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of 20mM TrisHCl, 100mM NaCl, pH 8.0.
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:GTF2B: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II. Associates with TFIID-IIA (DA complex) to form TFIID-IIA- IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV Vpr. Interacts with GPBP1. Interacts with Epstein-Barr virus EBNA2. Belongs to the TFIIB family.
UniProt Protein Details:

Protein type:Acetyltransferase; Nuclear receptor co-regulator; Transcription factor

Chromosomal Location of Human Ortholog: 1p22-p21

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function:protein binding; thyroid hormone receptor binding; transcription factor binding

Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

NCBI Summary:This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
UniProt Code:Q00403
NCBI GenInfo Identifier:135629
NCBI Gene ID:2959
NCBI Accession:Q00403.1
UniProt Secondary Accession:Q00403,Q5JS30, A8K1A7,
UniProt Related Accession:Q00403
Molecular Weight:34,833 Da
NCBI Full Name:Transcription initiation factor IIB
NCBI Synonym Full Names:general transcription factor IIB
NCBI Official Symbol:GTF2B
NCBI Official Synonym Symbols:TF2B; TFIIB
NCBI Protein Information:transcription initiation factor IIB
UniProt Protein Name:Transcription initiation factor IIB
UniProt Synonym Protein Names:General transcription factor TFIIB; S300-II
Protein Family:Transcription initiation factor
UniProt Gene Name:GTF2B
UniProt Entry Name:TF2B_HUMAN
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