Recombinant Human VDR/NR1I1 Protein (His Tag) (RPES4102)

SKU:
RPES4102
€402

Description

ELISA Kit Technical Manual

Recombinant Human VDR/NR1I1 Protein (His Tag)

Background:VDR (vitamin D(1,25- dihydroxyvitamin D3)receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1,25(OH)2D3) and induce expression of the 1,25(OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.
Name:Recombinant Human VDR/NR1I1 Protein (His Tag)
Size:20µg
Assay Genie SKU:RPES4102
Synonyms:NR1I1;PPP1R163
Species:Human
Expression host:Baculovirus-Insect Cells
Sequence:Met 1-Ser 427
Accession:P11473
Mol Mass:50 KDa
AP Mol Mass:50 kDa
Tag:C-His
Purity:> 88 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol
Reconstitution:Please refer to the printed manual for detailed information.
UniProt Protein Function:VDR: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Defects in VDR are the cause of rickets vitamin D- dependent type 2A (VDDR2A). A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Belongs to the nuclear hormone receptor family. NR1 subfamily.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: nucleoplasm; nucleus; receptor complex

Molecular Function:retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; vitamin D3 receptor activity; transcription factor activity

Biological Process: lactation; transcription initiation from RNA polymerase II promoter; positive regulation of apoptosis involved in mammary gland involution; cell morphogenesis; decidualization; negative regulation of transcription from RNA polymerase II promoter; signal transduction; intestinal absorption; cellular calcium ion homeostasis; negative regulation of cell proliferation; calcium ion transport; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; gene expression; negative regulation of transcription, DNA-dependent; skeletal development; positive regulation of keratinocyte differentiation

Disease: Vitamin D-dependent Rickets, Type 2a; Osteoporosis

NCBI Summary:This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]
UniProt Code:P11473
NCBI GenInfo Identifier:137617
NCBI Gene ID:7421
NCBI Accession:P11473.1
UniProt Secondary Accession:P11473,Q5PSV3, B2R5Q1, G3V1V9,
UniProt Related Accession:P11473
Molecular Weight:
NCBI Full Name:Vitamin D3 receptor
NCBI Synonym Full Names:vitamin D (1,25- dihydroxyvitamin D3) receptor
NCBI Official Symbol:VDR
NCBI Official Synonym Symbols:NR1I1; PPP1R163
NCBI Protein Information:vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; vitamin D nuclear receptor variant 1; nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163
UniProt Protein Name:Vitamin D3 receptor
UniProt Synonym Protein Names:1,25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
Protein Family:Vitamin D3 receptor
UniProt Gene Name:VDR
UniProt Entry Name:VDR_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
Baculovirus-Insect Cells
Reactivity:
Human
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