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SARS-CoV-2 NSP12 Recombinant Protein

SKU:
RPES7053
Product Type:
Recombinant Protein
Species:
Virus
€659
Frequently bought together:

Description

Product Name:SARS-CoV-2 NSP12 Recombinant Protein
Product Code:RPES7053
Size:50µg
Species:SARS-CoV-2
Expression Host:E.coli
Synonyms:SARS-CoV 2 nsp12,RNA-directed RNA polymerase,RdRp
Mol Mass:64kDa
AP Mol Mass:60 kDa
Tag:N-Gst
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin Level:Please contact us for more information.
Bio Activity:Testing in progress
Sequence:Asp4891-Val5212
Accession:QHD43415.1
Storage:Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Supplied as solution form in PBS, pH7.5 or lyophilized from PBS, pH7.5 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution:Please refer to the printed manual for detailed information.
Background:The RdRp of SARS-CoV-2 is composed of a catalytic subunit known as nsp12 as well as two accessory subunits, nsp8 and nsp7. The structure of this RdRp has recently been reported; it is highly similar to the RdRp of SARS-CoV, a zoonotic coronavirus that spread into the human population in 2002. The nsp12 subunit contains an N-terminal nidovirus RdRp-associated nucleotidyltransferase (NiRAN) domain, an interface domain and a C-terminal RdRp domain. The RdRp domain resembles a right hand, comprising the fingers, palm and thumb subdomains that are found in all single-subunit polymerases. Subunits nsp7 and nsp8 bind to the thumb, and an additional copy of nsp8 binds to the fingers domain. Structural information is also available for nsp8–nsp7 complexes.

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