The ABCF2 Antibody (CAB4365) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Related pseudogenes have been identified on chromosomes 3 and 7. RRID AB_2765643 Gene ID 10061 Swiss Prot Synonym ABC28; HUSSY18; HUSSY-18; EST133090; ABCF2
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
ABCF2 Antibody
SKU:
CAB4365
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ABC28, HUSSY18, HUSSY-18, EST133090, ABCF2
Positive Sample:
293T, HeLa, MCF7, Mouse testis, Mouse brain
Cellular Localization:
-
Calculated MW:
71kDa
Observed MW:
72kDa
This gene encodes a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Related pseudogenes have been identified on chromosomes 3 and 7. RRID AB_2765643 Gene ID 10061 Swiss Prot Synonym ABC28; HUSSY18; HUSSY-18; EST133090; ABCF2
Purification Method:
Affinity purification
Gene ID:
10061
RRID:
AB_2765643
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ABCF2 Rabbit pAb (CAB4365) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Immunofluorescence analysis of U2OS cells using ABCF2 Rabbit pAb (CAB4365) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
