The Actin (plant specific) Rabbit pAb (CABC053) is a high-quality antibody developed for reliable detection and analysis of target proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
This antibody is validated for use in WB applications and has demonstrated reactivity against Widely Range, Oryza sativa samples.
Product Name:
Actin (plant specific) Rabbit pAb
SKU:
CABC053
Size:
100μL, 20μL, 200μL, 50μL
Reactivity:
Widely Range, Oryza sativa
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WB
Recommended Dilution:
WB
,1:1000 - 1:5000
Synonyms:
ACT12, Actin(Plant Specific)
Positive Sample:
Oryza sativa
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
45kDa
Observed MW:
45kDa
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Purification Method
Affinity purification
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Oryza sativa using plant actin Rabbit pAb (CABC053) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:(ECL Basic Kit (AbGn00020)). Exposure time: 180s.
