The AIM2 Antibody (CAB3356) is a high-quality antibody developed for reliable detection and analysis of target proteins. AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. RRID AB_2765071 Gene ID 9447 Swiss Prot Synonym PYHIN4; AIM2
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
AIM2 Antibody
SKU:
CAB3356
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF
/
ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PYHIN4, AIM2
Positive Sample:
HT-29, Mouse spleen
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
39kDa
Observed MW:
39kDa
AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. RRID AB_2765071 Gene ID 9447 Swiss Prot Synonym PYHIN4; AIM2
Purification Method:
Affinity purification
Gene ID:
9447
RRID:
AB_2765071
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using AIM2 Rabbit pAb (CAB3356) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of A-431 cells using AIM2 Rabbit pAb (CAB3356) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
