The ANT1/ANT2/ANT3/ANT4 Antibody (CAB15988) is a high-quality antibody developed for reliable detection and analysis of target proteins. ANTs are the mitochondrial carrier subfamily of solute carrier proteins. ANT protein functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ANT1/ANT2/ANT3/ANT4 Antibody
SKU:
CAB15988
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
ANTs are the mitochondrial carrier subfamily of solute carrier proteins. ANT protein functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane.
Purification Method
Affinity purification
Gene ID
291 292 293 83447
RRID
AB_2763427
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ANT1/ANT2/ANT3/ANT4 Rabbit pAb (CAB15988) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 150s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ANT1/ANT2/ANT3/ANT4 Rabbit pAb (CAB15988) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ANT1/ANT2/ANT3/ANT4 Rabbit pAb (CAB15988) at dilution of 1:50 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
